Retraction for Anderson et al., "Assembly of Minicellulosomes on the Surface of Bacillus subtilis".
نویسندگان
چکیده
To cost-efficiently produce biofuels, new methods are needed to convert lignocellulosic biomass into fermentable sugars. One promising approach is to degrade biomass using cellulosomes, which are surface-displayed multicellulase-containing complexes present in cellulolytic Clostridium and Ruminococcus species. In this study we created cellulolytic strains of Bacillus subtilis that display one or more cellulase enzymes. Proteins containing the appropriate cell wall sorting signal are covalently anchored to the peptidoglycan by coexpressing them with the Bacillus anthracis sortase A (SrtA) transpeptidase. This approach was used to covalently attach the Cel8A endoglucanase from Clostridium thermocellum to the cell wall. In addition, a Cel8A-dockerin fusion protein was anchored on the surface of B. subtilis via noncovalent interactions with a cell wall-attached cohesin module. We also demonstrate that it is possible to assemble multienzyme complexes on the cell surface. A three-enzyme-containing minicellulosome was displayed on the cell surface; it consisted of a cell wall-attached scaffoldin protein noncovalently bound to three cellulase-dockerin fusion proteins that were produced in Escherichia coli. B. subtilis has a robust genetic system and is currently used in a wide range of industrial processes. Thus, grafting larger, more elaborate minicellulosomes onto the surface of B. subtilis may yield cellulolytic bacteria with increased potency that can be used to degrade biomass.
منابع مشابه
Assembly of Minicellulosomes on the Surface of Bacillus subtilis †
Assembly of Minicellulosomes on the Surface of Bacillus subtilis † Timothy D. Anderson, Scott A. Robson,‡ Xiao Wen Jiang, G. Reza Malmirchegini, Henri-Pierre Fierobe, Beth A. Lazazzera, and Robert T. Clubb* Department of Chemistry and Biochemistry, UCLA-DOE Institute for Genomics and Proteomics, Molecular Biology Institute, and Department of Microbiology, Immunology and Molecular Genetics, Univ...
متن کاملEngineering microbial surfaces to degrade lignocellulosic biomass
Renewable lignocellulosic plant biomass is a promising feedstock from which to produce biofuels, chemicals, and materials. One approach to cost-effectively exploit this resource is to use consolidating bioprocessing (CBP) microbes that directly convert lignocellulose into valuable end products. Because many promising CBP-enabling microbes are non-cellulolytic, recent work has sought to engineer...
متن کاملRetraction for Anderson et al., Recombinant Bacillus subtilis That Grows on Untreated Plant Biomass.
متن کامل
Production of minicellulosomes from Clostridium cellulovorans in Bacillus subtilis WB800.
Two genes encoding EngB endoglucanase and mini-CbpA1 scaffolding protein of Clostridium cellulovorans were constructed and coexpressed in Bacillus subtilis WB800. The resulting minicellulosomes were isolated by gel filtration chromatography and characterized. Biochemical and immunological evidence indicated that fraction II contained minicellulosomes consisting of mini-CbpA1 and EngB. The in vi...
متن کاملSynthesis of Clostridium cellulovorans minicellulosomes by intercellular complementation.
The ability of two strains of bacteria to cooperate in the synthesis of an enzyme complex (a minicellulosome) was examined. Three strains of Bacillus subtilis were constructed to express Clostridium cellulovorans genes engB, xynB, and minicbpA. MiniCbpA, EngB, and XynB were synthesized and secreted into the medium by B. subtilis. When the strains with the minicbpA and engB genes or with xynB we...
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ورودعنوان ژورنال:
- Applied and environmental microbiology
دوره 77 14 شماره
صفحات -
تاریخ انتشار 2011